By: Nasrullah I1, Murhandini S1, Rahayu WP1,2
1Research Center for Drug and Food, National Agency for Drug and Food Control
2Department of Food Science and Technology, Bogor Agricultural University
Curcuma aeruginosa Roxb.rhizome is empirically used as a traditional medicinefor rheumatic, cough, asthma and anthelmintic. To ensure quality of its extract, fingerprint/phytochemical study is needed to identify and standardize it.In this research, the phytochemical study was covered by material preparation and extraction from its crude extract, then followed by identification using TLC (Thin Layer Chromatography) scanner.From the results, n-hexane extract showed better separation in two times elution with n-hexane: ethyl acetate (9:1 v/v) and had specific retention time at 0.16, 0.30 and 0.48 (254 nm) and 0.26 (366 nm). Otherwise, clear separation of ethyl acetate extract was shown in chloroform: methanol (9:1 v/v) with specific retention time at 0.20, 0.39, 0.56 and 0.65 (254 nm) and 0.60 (366 nm). In conclusion, the specific retention time from both extracts can be used as fingerprint for standardization of traditional medicine extractof Curcuma aeruginosa Roxb. rhizome.
Keywords:Curcuma aeruginosa Roxb. rhizome, TLC, scanner, fingerprint, retention time
Presented at Journal of International Environmental Application and Science Volume 5, No.5. December 2010. ISSN-1307-0428