{Abstract} STABILITAS FOTOOKSIDASI MINYAK GORENG SAWIT YANG DIFORTIFIKASI DENGAN MINYAK SAWIT MERAH (Photo oxidation Stability of palm oil fortified by red palm oil)

By: Andarwulan N1,2, Muhammad GN1, Agista AZ 1, Dharmawan S 1, Fitriani D 1, Wulan AC 1, Pratiwi DG 1, Rahayu WP 1,2, Martianto D2,3, Hariyadi P 1,2

1Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University
2SEAFAST Center, Bogor Agricultural University, Bogor Indonesia
3Department of Nutrition Science, Faculty of Human Ecology, Bogor Agricultural University

Deterioration of palm oil fortified with vitamin A and pro vitamin A could be caused by the presence of oxygen and light exposure. The purpose of this study was to determine the effect of the initial peroxide value (PV) in palm oil (1.99, 3.98, and 9.95 meq O2/kg oil) and light intensity (15000, 10000, and 5000 lux) on the rate of oxidation and the shelf life of palm oil fortified with Red Palm Oil (RPO) equal to 45 IU vitamin A. The RPO contained β-carotene as pro vitamin A in the amount of 504.67 ppm. The PV and free fatty acid (FFA) content were observed as the parameters of oil deterioration during storage. The results showed that the rate of PV was influenced by light intensity, while the rate of FFA formation were more influenced by the amount of initial PV in the oil. Based on the palm oil standard quality for PV (SNI 7709: 2012), the shelf life of palm oil with the lowest initial PV at ambient temperature was 9.5 days, while that with the highest PV was 1.32 hours. The deterioration rate of RPO fortified palm oil due to light exposure was also compared with its deterioration rate due to heat and the deterioration rate of palm oil fortified with vitamin A. The shelf life of vitamin A fortified palm oil stored in the dark was 90.67 days, while RPO fortified palm oil was 68.12 days. This shelf life results showed that RPO had a potency as pro vitamin A fortificant for palm oil as long as it is stored in a closed container in the dark.
Keywords: palm oil; peroxide value; photooxidation; red palm oil

Published at Jurnal Teknol. dan Industri Pangan 27 (1): 31-39. 2016. ISSN 1979-7788

{Abstract} IDENTIFIKASI LISTERIA SPP. PADA PANGAN JAJANAN BERBASIS IKAN DI KOTA BOGOR (Identification of Listeria spp in fish –based snack in Bogor)

By: Yuswita E1, Nurjanah S1,2, Rahayu WP1,2

1Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University
2SEAFAST Center, Bogor Agricultural University, Bogor Indonesia

Listeria monocytogenes contamination in processed food, especially fish-based snack, may results from contaminated raw materials, underprocessed or recontamination. The aims of this study were to identify the presence of Listeria spp. especially L. monocytogenes by PCR method and biochemical methods, as well as calculate the prevalence of Listeria spp. in fish-based snack food in Bogor. This study was conducted of 4 steps: (1) determination of L. monocytogenes’s DNA limit detection, (2) sample preparation, (3) identification of L. monocytogenes with real-time PCR, and (4) identification of Listeria spp. with biochemical methods. The results showed that DNA detection limits of L. monocytogenes in fish meatball and otak-otak were at 8.3×102 and 2.9×102 CFU/g, respectively. The study on 65 samples indicated that contamination of L. monocytogenes was not observed, but other species of Listeria spp., namely L. grayi and L. innocua, were found. The prevalence of L. grayi and L. innocua in siomay was at 5.9%, while the prevalence of L. grayi in shrimp meatballs, fish meat balls, fried meatballs was at 8.3, 9.1, and 50%, respectively. Furthermore, L. innocua from takoyaki samples with a prevalence of 20% was observed.

Keywords: fish-based snack; Listeria spp.; L. monocytogenes

Published at Jurnal Teknologi dan Industri Pangan 27(1): 10-16. 2016. Doi : 10.6066/jtip.2016.27.1.10.

{Abstract} IDENTIFICATION OF LISTERIA MONOCYTOGENES ON GREEN MUSSELS AND COCKLE SHELL

By: Rahayu WP1,2, Rinanti R 1, Nurjanah S1,2, and Nurwitri CC1

1Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University
2SEAFAST Center, Bogor Agricultural University, Bogor Indonesia

Green mussel (Perna viridis) and cockle shell (Anadara granosa) are one of many sources of animal protein which is many cultivated in Indonesia because their price is relatively affordable. This study was conducted to identify the presence of Listeria monocytogenes in 27 samples of green mussels and 3 samples of cockle shells using real-time Polymerase Chain Reaction (real-time PCR) and biochemical methods. The target gene for amplification in real-time PCR was an hlyA gene because this gene was a determinant of virulence genes that produce listeriolysin O. Primers used in this study were forward primer DG69 (GTG CCG GGT AAA AGA CCA TA) and reverse primer DG74 (CGC CAC TGA GAT ACT AT) and fluorescence signals indicator using SYBR Green I. The results of analysis using real-time PCR were negative Listeria monocytogenes in all samples, while using biochemical methods there was one of 30 samples contaminated by Listeria welshimeri.

Keywords: hlyA gene, Listeria monocytogenes, Listeria spp., mussel, real-time PCR

Published at Jurnal Pengolahan Hasil Perikanan Indonesia 19(3): 329-338. 2016
DOI: 0.17844/jphpi.2016.19.3.329

{Abstract} PHYSICOCHEMICAL AND STABILITY OF GOAT CHEESE WITH MONO AND MIXED CULTURE OF LACTOBACILLUS PLANTARUM AND LACTOBACILLUS RHAMNOSUS

By: Setyawardani T1, Rahayu WP2, Palupi NS2

1 Department of Animal Production, Faculty of Animal Science, University of Jenderal
Soedirman Purwokerto 53123, Indonesia
2 Department of Food Science and Technology, Faculty of Agricultural Engineering and
Technology, Bogor Agricultural University, Bogor 16002, Indonesia

The purpose of the study is to assess the effect of the use of mono probiotic culture and mixed cultures of Lactobacillus plantarum and Lactobacillus rhamnosus on the physicochemical properties and its viability during storage. The soft cheeses were made through three treatments: (1) the use of Lactobacilllus rhamnosus culture, (2) the use of Lactobacillus plantarum culture and (3) the use of mixed cultures (Lactobacillus rhamnosus and Lactobacillus plantarum). The variables measured were the cheese texture (firmness and stickiness), proximate, pH of the product, and the number of LAB. The results showed that cheese firmness ranged from 10.78 to 47.75 gf, cheeses stickiness was -8.23 to -11.53 gs, cheese pH was 4.70- 5.60; number of cheese LAB was 8.59-9.69 log CFU/g. The content of protein, fat, and ash were 13.65-16.54, 15.28-20.03, and 2.7-3.39%, respectively. The conclusion of this study was that the soft cheeses with mixed cultures of L.rhamnosus and L.plantarum are potentially good to be developed as a probiotic food.

Keywords: Soft cheese, Lactobacillus rhamnosus, Lactobacillus plantarum, Culture

Published at Animal Production 18 (1): 36-42. 2016. ISSN 1411-2027

{Abstract} PENERAPAN GOOD LOGISTIC PRACTICES UNTUK PRODUK PERIKANAN (Good Logistic Practices Application on Fish Product)

By: Rahayu WP1 and Adhi W2
1 Department of Food Science and Technology, Bogor Agricultural University
2 Alumni Department of Industrial Technology. Bogor Agricultural University

The utilization of Indonesia’s fisheries potential should be supported by the development of fisheries industry, more specifically by the improvement on fisheries supply chain management. It is needed to preserve the quality and safety of fisheries product from sea to consumers’ table and sustainable fisheries supply. The fisheries product is perishable, moreover the appropriate handling and full controlled process are needed to maintain product quality and safety. In fisheries industry, quality and safety can be maintained by applying good logistic practices (GLoP). GLoP is best practices of cold chain management combined by quality and safety control system such as good manufacturing practices (GMP), standard sanitation operating procedure (SSOP) and hazard analysis critical control point (HACCP). On the other hand, the sustainability of fisheries supply can be achieve by applying full controlled exploitation process on the resources. With commitment and cooperation of all parties on fisheries industry, the standard of fisheries industry can be improve gradually and it is necessary to improve quality and quantity of Indonesia’s fisheries products.

Keywords: cold chain management, good logistic practices, GMP, SSOP, sustainable fisheries.

Published at Jurnal Manajemen Transportasi dan Logistik 2 (1): 129-147. 2016. ISSN 2355-4721

{Abstract} IDENTIFICATION AND PROBABILITY OF ILLNESS OF S. AUREUS CONTAMINATED FOOD FOR SCHOOL CHILDREN

By: Yunita NA1, Rahayu WP2,3, Suliantari2,3, Nurjanah S2,3, and Nurwitri CC2

1Study program of Food Science, Bogor Agricultural University,
2Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University
3SEAFAST Center, Bogor Agricultural University, Bogor Indonesia

Food for school children holds an important role in the fulfillment nutritional intake of children at the school age, therefore it has to be safe for consumption. The research that had been conducted in Bogor to thirty five samples from twenty two food sellers in eight elementary schools in two different time periods. The analysis results showed that none of the samples was contaminated by L. monocytogenes, Salmonella spp or Vibrio spp. Two samples (otak-otak and siomai) were contaminated by S. aureus. The contamination source tracing showed that the hands, equipment, and environment around the selling place were contaminated by S. aureus. The likelihood of illness by S. aureus after consuming food and snack for school children was 0.00103 or one case for every 972 servings.

Keywords: food for school children, pathogenic bacteria, probability of illness, S. aureus.

Published at Int. Food Res. Journal 23 (4): 1767-1772. 2016. ISSN 19854668/e:22317546

{Abstract} DETECTION OF LISTERIA MONOCYTOGENES IN INDIGENOUS SNACK USING REAL-TIME POLYMERASE CHAIN REACTION

By: Mutsyahidan AMA1, Rahayu WP2,3, Nurjanah S2,3

1Department of Agricultural Product Technology, Polytechnic of Gorontalo, Indonesia
2Department of Food Science and Technology, Bogor Agricultural University
3SEAFAST Center, Bogor Agricultural University

Listeria monocytogenes is an important foodborne pathogen that can cause serious human listeriosis. The aim of this study was to optimize real-time PCR method for L. monocytogenes detection in indigenous Indonesian snack (pempek). DNA isolation of L.monocytogenes was conducted by comparing three extraction methods: phenol:chloroform, heating, and commercial kit (QIAamp DNA Blood Mini Kit). DNA isolate was amplified by PCR using two variables, which were LIM 2 and LIMRE primers for iap gene detection, and DG69 and DG74 primers for hlyA gene detection. Phenol:chloroform method showed the best extraction result, while DG69 and DG74 primers showed more specific result on PCR compared with LIM 2 and LIMRE primers. The running conditions gave specific amplification curve until 27th cycle. DNA detection in L. monocytogenes culture generated standard curve equation of Ct = 37.9 – 3.11 C with limit of detection (LOD) at 3.2 x 103 CFU/mL, while DNA detection in pempek generated standard curve equation of Ct = 41.03 – 3.69 C with limit of detection (LOD) at 6.3 x 103 CFU/g. Real-tie PCR with DG69 and DG74 primers could be considered as a reliable method for specific and sensitive detection of L. monocytogenes.

Keywords : Detection, Indigenous snack, Listeria monocytogenes, Real-time PCR.

Published at Malaysian Journal of Microbiology. 12(2): 177-181. 2016. ISSN (print) 1823-8262, ISSN (online): 2231-7538

{Abstract} ESTIMATION OF ECONOMIC LOSS DUE TO FOOD POISONING OUTBREAKS

By: Rahayu WP1,2, Fardiaz D1, Kartika GD1, Nababan H3, Fanaike R3, Puspitasari R3

1Department of Food Science and Technology, Bogor Agricultural University
2SEAFAST Center, Bogor Agricultural University,
3National Agency for Drug and Food Control, Jakarta 10560, Indonesia

Food poisoning outbreaks frequently occur in many countries resulting in economic loss. A formula for calculation of economic loss due to food poisoning outbreaks in Indonesia was developed. Estimation of the loss was developed based on experiences in several countries. Related data were also collected from local health facilities, food industry officials, and other sources to improve the accuracy of the formula. Calculation of the economic loss was performed based on cases of food poisoning outbreaks recorded in 2013. Economic loss was estimated based on direct health-care costs, direct non-health-care costs, and indirect non-health-care costs. The economic loss of food poisoning outbreaks in Indonesia in 2013 was approximately US$ 78 million. The high-cost of economic loss due to food poisoning outbreaks suggests a need for additional and serious efforts for controlling all aspects of food safety.

Keywords: outbreak, estimate of economic losses, food poisoning, food safety, Indonesia

Published at Food Science and Biotechnology 25(s): 157-161. 2016. DOI 10-1007/s10068-016-113-8

{Abstract} POLA KONSUMSI PANGAN IBU PASCA MELAHIRKAN DI RSIA THAHA BAKRIE SAMARINDA (Postpartum Food Consumption Pattern at RSIA Thaha Bakrie Samarinda)

By: Ramadhani AS1, Astawan M2, Rahayu WP2

1 Program Studi Magister Profesional Teknologi Pangan, Sekolah Pasca Sarjana, Institut Pertanian Bogor
2 Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor

Food taboos are practiced in some Indonesian culture. For example there is a tendency that postpartum mother is not recommended to consume protein sources in Samarinda, East Kalimantan. The objective of this study was to evaluate food intake of postpartum mothers, including variety of food taboos which it can affect to mothers. The research involved 40 postpartum respondents, age 19-40 years. Respondents were interviewed using a questionnaire and food recall form. Analysis of data is Uni-variate and Bi-variate analysis, bivariate analysis using chi-square statistical test with 90% level of significance (α=0.10). The result showed 65% of respondents had taboos to certain foods. The most that 80% avoided food was seafood. Factors that affect food taboos from the neighborhood and education level of respondents.

Keywords: Food abstinence, food recall, postpartum mother
Published at Jurnal Mutu Pangan 2(2): 136-143, 2015. ISSN 2355-5017

{Abstract} PERUBAHAN SIKAP KEAMANAN PANGAN SISWA BERDASARKAN PERSEPSI ORANG TUA DAN ANAK (Food Safety Attitude Alteration of School Children based on Parents and Students Perspective)

By: Rahayu WP 1,2, Nurulfalah S1, and Fanaike R3

1 Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor
2 South East Asian Food and Agricultural Science and Technology Center, Institut Pertanian Bogor
3 Badan Pengawas Obat dan Makanan Republik Indonesia

Food safety program in school is one of effort to keep children from the risk of consuming unsafety foods. Food safety programs that applied in school were posting food safety poster, conducting food safety extension, establishing food safety team, operating food safety car, and accessing klub pompi websites. This research was aim to gain knowledge of school children’s attitude alteration after receiving food safety program and parents support in food safety program in school. The survey was conducted to 397 children and 195 parents, and the results were processed by SPSS program using correlation and contingency analysis. The survey results showed the activities that give significant effect to attitude alteration of school children in the West part of Indonesia were adhering poster and establishing food safety team in school. Meanwhile posting food safety poster and conducting food safety extension in school have given significant effect in the East part of Indonesia. Attitude alteration of school children to buy food at school canteen than at food vendors outside school canteen is in high category in the West part of Indonesia (66%) and very high in the East part of Indonesia (94%).

Keywords: Food safety, parent, primary school student, program.

Published at Jurnal Mutu Pangan 2(2): 112-118, 2015. ISSN 2355-5017