By: Rahayu D1, Rahayu WP2,3, Lioe HN2, Herawati D2,3, Broto W4, Ambarwati S5
1Program Studi Ilmu Pangan, Fakultas Teknologi Pertanian-IPB
2Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian-IPB
3Seafast Center, Fakultas Teknologi Pertanian-IPB
4Balai Besar Penelitian dan Pengembangan Pasca Panen Pertanian
Fusarium verticillioides was the predominant species in producing fumonisin on agricultural products. Fumonisisn B1 (FB1) is the most abundant fumonisin in nature and the most toxic than other fumonisin. The main factors affecting the growth of and production of fumonisin are temperature and humidity. This research aimed to assess the effect of changes in temperature and humidity on the growth of and FB1 production on maize and soybeans medium. Maize and soybeans that have inoculated with suspension Bio 957 were incubated at 20, 30 and 40 °C with 70, 80 and 90% of humidity for 14 days. Observations of growth made by weighing the cells mass and analysis of FB1 production performed by HPLC. The results showed that the highest growth of Bio 957 in maize and soybeans was occurred at temperature 30 °C and 90% of humidity, the cell mass weights were 904,5 and 885,5 mg per 20 g of maize and soybeans respectively. The highest concentration of FB1 in maize and soybeans were 374 and 67 pbb respectively, observed at temperature 30 °C for maize and 20 °C for soybeans, both at same humidity (90%). The results showed that Bio 957 was able to grow well and produced the highest concentrations of FB1 in maize and soybeans at a temperature of 20 and 30 °C with 90% of humidity. At a temperature of 40 °C with 70, 80 and 90% of humidity, the growth of was not observed, therefore FB1 formation was avoided.
Keywords: Fumonisin B1, Bio 957, humidity, temperature
Telah diterbitkan di: Jurnal Agritech. 2015. 35 (2): 156-163