{Abstract} AKTIVITAS ANTIAFLATOKSIN B1 EKSTRAK DAUN RUMPUT KEBAR (Biophytum petersianum) TERHADAP Aspergillus flavus (Antiaflatoxin B1 Activity of Kebar Grass (Biophytum petersianum) Leaf Extract on Aspergillus flavus)

By: Lisangan MM1,2, Syarief R3, Rahayu WP3,4, Dharmaputra OS5,6

1Study program of Food Science, Bogor Agricultural University
2Faculty of Agriculture and Agricultural Technology, The State University of Papua
3Faculty of Agricultural Engineering and Technology, Bogor Agricultural University,
4Southeast Asia Food and Agriculture Science and Technology Center, Bogor Agricultural University
5Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
6Southeast Asian Regional Centre for Tropical Biology (SEAMEO BIOTROP), Jl. Raya Tajur, Bogor, West Java-Indonesia

Aflatoxin B1 was a secondary metabolite produced by Aspergillus flavus having negative effect on human health because of its carcinogenic. Many efforts have been done to investigate the antifungal and antiaflatoxin agent derived plant. The objective of this research was to study the activity of antifungal from kebar grass leaf extract on mycelial growth and aflatoxin B1 production of Aspergillus flavus BCC F0219 and A. flavus BIO 2236 isolates in food model medium i.e. carbohydrate-enriched medium, fat-enriched medium and protein-enriched medium. Kebar grass leaf extracts was successively obtained by using n-hexane – ethyl acetate – methanol (HEM). Concentrations of the extract tested on A. flavus BCC F0219 and A. flavus BIO 2236 were 1; 1.5, and 2 MIC. The MIC for A. flavus BCC F0219 in carbohydrate-enriched medium, fat-enriched medium, and protein-enriched medium were 12, 14, and 14 mg/mL, respectively. Meanwhile, the MIC for A. flavus BIO 2236 in carbohydrate-enriched medium, fat-enriched medium and protein-enriched medium were 12, 16 and 16 mg/mL, respectively. The results showed that the percentage of growth inhibition of A. flavus BCC F0219 and BIO 2236 in carbohydrate, fat and protein-enriched medium at 3 different levels of MIC concentrations ranged between 90.8 – 100% and 93.8 – 100%. The inhibitory effect of Aflatoxin B1 production of A. flavus F0219 BCC and BIO 2236 in carbohydrate, fat and protein-enriched medium at 3 different levels of MIC concentration ranged between 70.86 – 100 % and 83.42 – 98.84 %.

Keywords: Aflatoxin B1, anti aflatoxin, Aspergillus flavus, Biophytum petersianum, food model medium, kebar grass

Telah diterbitkan di Jurnal Agritech. 2015. 35 (1): 116-128

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