{Abstract} POLA PERTUMBUHAN Aspergillus ochraceus BIO 220 DAN PRODUKSI OKRATOKSIN A PADA JAGUNG DAN KEDELAI INVITRO (Growth Pattern of Aspergillus ochraceus and Ochratoxin A Production on Maize and Soybeans Invitro)

By:Simatupang S<sup</,1 Rahayu WP2,3, Lioe HN2, Herawati D2,3, Broto W<sup<4, Ambarwati S5

1Program Studi Ilmu Pangan-Institut Pertanian Bogor
2Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian- Institut Pertanian Bogor
3SEAFAST Center- Institut Pertanian Bogor
4Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian Bogor

Toxigenic fungi, Aspergillus ochraceus producing ochratoxin A can cause serious health problem if the fungi contaminated food product such as maize and soybean. A. ochraceus growth is affected by climate change including the change of temperature and relative humidity. This study was performed to evaluate the effect of temperature and relative humidity on A. ochraceus BIO 220 growth and its ochratoxin A production. Aspergillus ochraceus BIO 220 was inoculated in Potatoes Dextrose Agar (PDA) media, maize and soybean, then incubated at 3 different temperatures (20, 30, 40 oC) dan 3 different relative humidity (70, 80 dan 90%) for 20 days. Mycelium and spores were observed every two days and the level of ochratoxin A was analyzed using RP-HPLC equipped by fluorescence detector after 40 days. Optimum growth for A. ochraceus BIO 220 in laboratory media, maize and soybean was at temperature 30 °C and relative humidity 90%, while the highest ochratoxin A level was reached in maize (93 ppb) and soybean (45 ppb) at temperature of 20 °C and 80 % relative humidity. Aspergillus ochracheus BIO 220 could not grow in maize and soybean at extreme condition (temperature 40 °C and relative humidity 70, 80 dan 90 %).

Keywords : Aspergillus ochraceus, ochratoxin, relative humidity, temperature

Dimuat di: Jurnal Pascapanen 11 (1): 48-56. 2014

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