{Abstract} BIOCONTROL OF TOXIGENIC MOLDS by Saccharomyces cerevisiae ATCC 9376

By Rahayu WP1,2, Herawati D1,2,  Broto W3,  Ambarwati S4,Lioe HN1, Simatupang S5,  Pratiwi C5,  Rahayu D1

1Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University/IPB,
2SEAFAST Center-IPB,
3Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture,
4SEAMEO BIOTROP,
5Study Program of Food Science-IPB

Toxigenic molds (Aspergillus flavus, Aspergillus ochraceus, and Fusarium verticillioides) are able to produce mycotoxin and contaminate crops (i.e. maize and soybean), which in the next may have serious impact to health risk. This research was designed to investigate the effectiveness of antagonist yeast, S. cerevisiae, as a biological competitor for the growth of toxigenic molds. The biocontrol of toxigenic molds on Potatoes Dextrose Agar (PDA), soybean and maize was conducted by growing toxigenic molds together with S. cerevisiae ATCC 9376 for 10 (A. flavus BIO 2237), 15 (A. ochraceus BIO 220), and 8 (F. verticillioides BIO 957) days. This research showed that S. cerevisiae ATCC 9376 could inhibit A. flavus BIO 2237, A. ochraceus BIO 220, and F. verticillioides BIO 957with the inhibition percentage: 46, 43, and 44 % on PDA medium; 47, 59, and 45 % on maize medium; and 44, 49, and 39 % on soybean medium respectively. The result showed that A. ochraceus BIO 220 become the most inhibit toxigenic molds among others and maize was the most effective media of inhibition. Furthermore, this preliminary information could be used for biological control of toxigenic molds growth and mycotoxin control in foods.

Keywords: maize, S. cerevisiae, soybean, toxigenic molds

The paper has been presented in International Conference on Food for A Quality Life. Jakarta, 15 – 16 October 2014

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