{Abstract} ANTIMICROBIAL ACTIVITY AND ADHESION ABILITY OF INDIGENOUS LACTIC ACID BACTERIA ISOLATED FROM GOAT MILK

By: Setyawardani T1, Rahayu WP2, Maheswari RR3, and Palupi NS2

1Department of Animal Production, Faculty of Animal Science, Jenderal Soedirman University, Indonesia
2Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, Campus IPB Darmaga, PO Box 220, Bogor 16002, Indonesia
3Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, Indonesia

To be categorized as probiotic bacteria, lactic acid bacteria (LAB) must have at least antimicrobial activity and adhesion ability on intestinal mucosal surfaces. The aim of this study was to determine the antimicrobial activity and adhesion ability of eight LAB strains isolated from goat milk. The isolates passed initial selection on low pH (2.0, 2.5, and 3.0) and bile salt tolerance (0.3% Oxgall). Three strains of L. rhamnosus (TW2, TW3, TW32); five strains of L. plantarum (TW4, TW10, TW14, TW26, and TW28) were examined for their antimicrobial activity against both spoilage and pathogenic bacteria (S. Typhimurium ATCC 14028, E. coli ATCC 8739, B. cereus ATCC 13061, S. aureus ATCC 25923 and P. aeruginosa ATCC 9027). Five isolates (TW2, TW4, TW14, TW28, and TW32) were evaluated for their ability to adhere to intestinal mucosal surfaces. A modified well diffusion method was used to assess the antimicrobial activity. A modification of an animal model was also used to analyze the LAB adhesion ability. The adhesion of the bacteria on jejunum and ileum was examined by using Scanning Electron Microscope. The result showed that the isolates had diameters of inhibition ranging from 12.6 to 19.9 mm for S. Typhimurium, 11.3 to 21.4 mm for E. coli, 7.5 to 19.9 mm for B. cereus, and 9.9 to 24.7 mm for P. aeruginosa. L. plantarum TW10 and TW26 had no inhibition activity for S. aureus. The adhesion ability of the bacteria was 0.54-2.19 log CFU/cm2 on the intestinal mucosal surfaces. The highest adhesive level in jejunum and ileum was showed by L. rhamnosus TW2.

Keywords: adhesion ability, antimicrobial, lactic acid bacteria, goat milk

Published at Int. Food Res. Journal 1(3): 959-964. 2014. ISSN 19854668/e:22317546

{Abstract} PEMENUHAN REGULASI PELABELAN PRODUK INDUSTRI RUMAH TANGGA PANGAN (IRTP) DI BOGOR (Fulfillment of Food Labeling Regulations for Small Medium Enterprises (SMEs) Products in Bogor)

By: Wijaya, WA and Rahayu WP

Department of Food Science and Technology, Bogor Agricultural University

A food label is an important tools for food trade activities, thus it required to be regulated and controlled so the information about the food that delivered to the public was true and not mislead. This research was a descriptive research with aimed to evaluate the degree of fulfillment of food product labels of Small Medium Enterprises (SMEs) in Bogor compared with existing regulations (Indonesian Act Number 18 of 2012). Samples were chose from three majority of SMEs products in Bogor, there were flour products and its processing; products from the process of grains and tubers; and softdrink and powdered beverages products. Four group of element were tested: 1) technical labeling, 2) format content, 3) minimum information, and 4) prohibited information. The results showed the level of fulfillment for those three types of products were: 1) technical labeling: 44, 45, and 73%, 2) format content: 75, 80, and 60%, 3) minimum information: 69, 64, and 66%; and 4) prohibited information: 99, 100, 96%, respectively. The average level of fulfillment for those three types of products were 72, 72, and 74%, respectively. This low result of level of fulfillment showed that it still need further development of SMEs, especially about food labelling criteria according to the existing regulations.

Keywords: Label, level of fulfillment, regulations, SMEs

Dimuat di Jurnal MUTU PANGAN 1(1): 67-73. ISSN 2355-5017. 2014.