By: Maya Purwanti1, Mirnawati Sudarwanto2, Winiati P Rahayu3, A. Winny Sanjaya2 (1Extension Livestock, Bogor Agricultural Extension Academic, 2Faculty of Veterinary Medicine, Bogor Agricultural University, 3Department of Food Science and Technology, Bogor Agricultural University)
The aim of this study was to evaluate the ability of Bacillus cereus and Clostridium perfringens spores to survived in different preparation temperature (25, 35 and 70oC for B. cereus and 25, 45, 70oC for C.perfringens) and modified storage condition (with 50 and 75% humidity; opened, closed and opened twice a day during period of observation) of powdered milk formula. The spores of B. cereus ATCC 13061 and C.perfringens CP-1 artificially contaminated to the milk formula. Result showed that B. cereus and C.perfringens spores were germinated and growth in reconstituted milk formula. Bacteria population increased ≥ 1 log within 3 hours at room temperature (28-29oC). The spores of both bacteria survived from dry condition of powdered milk formula, like when aw of the formulas increased cause of storage condition.
Keywords: Bacillus cereus, Clostridium perfringens, powdered milk formula, preparation temperature, storage condition.
Dimuat di Jurnal Teknologi dan Industri Pangan, Volume 20 No. 1, 2009, ISSN 1979-7788