By: Parhusip AJN1, Jennie BSL2, Yasni S2, Rahayu WP2
1Department of Food Technology – Faculty of Industrial Technology, UPH
2Department of Food Science and Technology-Bogor Agricultural University
Anti bacterial extract of andaliman extract is highly affected by its polarity. Ethyl acetate and methanol extract showed inhibition activity toward the growth of Stapylococcus aureus, while hexane extract did not. Ethyl acetate extract showed larger inhibition area compared to methanol extract. Ethyl acetate extract has higher ability to inhibit the growth of Staphylococcus aureus during its exponential phase compared to stationary or lag phase. The inhibition area itself ranges from 13.12 to 24.14mm when the concentration applied is between 10 to 50% (w/w). Ethyl acetate extract also gave lower MIC and MBC value (1.20 and 2.80%, respectively) compared to methanol extract (1.60 and 5.00%, respectively). Inhibition area of ethyl acetate and methanol extract at 4000 µg/ml is 15.69 ± 0.71and 11.13 ± 0.14 mm. When compared to antibiotics (streptomycin, penicillin G and polymiksin B), both ethyl acetate and methanol extracts were half less powerful.
Keywords: antibacterial, solvent, extract, cell
Dipresentasikan pada Seminar PATPI, Jakarta-Indonesia, November 3-4th 2009.
Proceeding ISBN 978-979-99570-5-4