Purwanti M1, Sudarwanto M2, Rahayu WP3, Sanjaya AW2
1Extension Livestock, Bogor Agricultural Extension Academic
2Faculty of Veterinary Medicine, Bogor Agricultural University
3Department of Food Science and Technology, Bogor Agricultural University
Through a nutritional improvement project, Bogor District had donated powdered milk formula (PMF) for severe malnutrition children less than five years. Powdered milk formula was assumed as an unsterilized product, because it might contain spore forming bacteria. To evaluate the bacterial contaminant of home prepared milk formula, forty eight samples of PMF, and 50 samples of reconstitution formula, drinking water, drinking equipment, and hand were taken proportionally from the mothers in 10 Health Community Centre around Bogor District. Samples were analyzed for aerobic microbe, Bacillus cereus, Cclostridium perfringens, and the potential of enterotoxin production. All of the donated PMF samples exhibiting a total aerobic count of < 104 CFU/g (mean 1.2 x 102 CFU/g) and B. cereus count of < 103 CFU/g (mean 3.0 x 102 CFU/g for PMF containing this bacterium). Improper home storage promoted the growth of aerobic microbe and B. cereus. Improper preparation promoted the growth of B. cereus which present initially at low level (mean 7.8 x 10 CFU/g) and became 1.6 x 103 CFU/ml after the reconstitution. Clostridium perfingens emerge at 4 samples (mean 1.5 x 10 CFU/ml) after the reconstitution. Several isolate of B. cereus (4 from PMF, 13 from opened PMF, and 2 from reconstruction formula) showed a possibility to produce diarrheagenic enterotoxin while the isolate of C. perfringens did not show it.
Keywords:powder milk formula, B. cereus, C. perfringens, diarrheagenic enterotoxin, preparation, storage
Dimuat di Forum Pascasarjana, Volume31 No. 4, 2008, ISSN 0126-1886