[abstract] SUB-CHRONIC TOXICITY TEST FOR CHITOSAN

By: Adriany R1, Rusmawati E1, Murhandini S1, Rahmi F1, Erlina T1, Murtiningsih1, Rahayu WP1,2

1Research Center for Drug and Food, National Agency for Drug and Food Control
2Department of Food Science and Technology, Bogor Agricultural University

Chitosan is a substance which is usually used in food and pharmaceutical industries, cosmetics, agriculture and environment as well. As a wide spread of using chitosan, it is important to have safety data especially the chitosan that is marketed in Indonesia. This research carried out 90 days sub chronic toxicity test in Wistar strain of male and female rats. This study was begun by making animal groups for 60 males and 60 females which were divided into control groups (water and acetic acid solution 1%), 3 treatment groups (sub chronic and satellite), with 10 and 5 animals per group of each. Treatment groups were respective daily oral administered by 100; 200; 400; mg chitosan in 1% acetic acid solution per kg body weight for 90 days. Sub chronic groups were autopsied a day after the last giving chitosan otherwise satellite groups were autopsied 30 days after the last giving chitosan. The result shows that dosing chitosan until 400 mg per kg body weight for 90 days did not affect clinical toxicity signing, depress body weight increasing, neither cause macro pathological disorder nor vital organ hystopathological disorder, and generally it did not cause hematological disorder as well as clinical biochemistry. Giving chitosan with dosing as mentioned above for 90 days can decrease blood totally cholesterol and low density lipid (LDL) amount and increase high density lipid (HDL) amount significantly compared to control group. Decreasing of totally cholesterol and increasing of HDL cholesterol were irreversible characteristic (permanently, did not influenced by stopping the test substance administration).

Keywords: chitosan, sub chronic toxicity

Presented at International Conference, Exhibition and Short Course on Nutraceuticals and Functional Foods, Bali-Indonesia, October 11-15th 2010

 

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