By: Iltizam Nasrullah, Sri Murhandini, Winiati P.Rahayu (Research Center for Drug and Food, National Agency of Drug and Food Control of the Republic of Indonesia, Jl. Percetakan Negara No 23, Jakarta 10560, Indonesia)
Sonchus arvensis L. leaves is empirically used as a traditional medicine for asthma, cough, anti-inflammation and diuretic. To ensure quality through identification and standardization of its extract, fingerprint/phytochemical study is needed. In this research, the phytochemical study was carried out by TLC (Thin Layer Chromatography) scanner and HPLC (High Performance Liquid Chromatography). From the results, n-hexane extract showed a better separation with toluen:ethyl acetate (93:7 v/v) and had specific retention factor 0.13; 0.31; 0.36; 0.46; 0.51 (254 nm) and 0.13; 0.32; 0.36; 0.46; 0.51; 0.59 (366 nm). Chloroform extract showed specific retention factor 0.13; 0.36; 0.46; 0.52 (254 nm) and 0.13; 0.32; 0.36; 0.46; 0.52; 0.61 (366 nm). Otherwise, clear separation of ethyl acetate extract was shown in chloroform:toluen: ethanol (4:4:1 v/v/v) with specific retention factor 0.23; 0.34; 0.36; 0.39; 0.80 (254 nm) and 0.20; 0.26; 0.34; 0.48; 0.76 (366 nm). From HPLC chromatogram at 254 nm, using acetonitrile-phosporic acid mixture showed specific retention time at 2.46; 4.09; 4.83; 7.69; 10.02; 11.06; 11.73 minute for hexane extract and 3.66; 5.84; 6.98 minute for ethyl acetate extract. In conclusion, the specific retention time from both extracts can be used as fingerprint for standardization of traditional medicine extract of Sonchus arvensis L. leaves.
Key words: Sonchus arvensis L. leaves, TLC scanner, HPLC, fingerprint, retention factor and retention time
Presented at 59th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research. Antalya, Turkey, 4-9 September 2011.