By: Iltizam Nasrullah1, Sri Murhandini1, Winiati P. Rahayu12 (1Research Center for Drug and Food, National Agency for Drug and Food Control, 2Department of Food Science and Technology, Bogor Agricultural University)
Piper retrofractum Vahl. fructus is empirically used as a traditional medicinefor diarrhea, cough, anti-oxidant, oxytoxic for post labor and carminative. To ensure quality through identification and standardization of its extract, fingerprint/phytochemical study is needed. In this research, the phytochemical study was carried out by TLC (Thin Layer Chromatography) scanner. From the results, with hexane:ethyl acetate (70:30 v/v), ethanol, ethyl acetate, chloroform and n-hexane extracts showed the same specific retention factorabout 0.20; 0.25; 0.29; 0.37; 0.69 (254 nm) and 0.20; 0.32; 0.36; 0.54; 0.68 (366 nm).With toluene:ethyl acetate (80:20 v/v), all extractsshowed specific retention factor about 0.25; 0.35; 0.48; 0.55; 0.61; 0.84 (254 nm) and 0.33; 0,44; 0,48; 0.55; 0.84(366 nm). In addition, with toluene:chloroform:ethyl acetate (60:30:10 v/v), all extracts showed specific retention factorabout 0.32; 0.36; 0.42; 0.52; 0.64; 0.77 (254 nm) and 0.32; 0.36; 0.51; 0.76 (366 nm). In conclusion, the specific retention time forall extracts with different eluent can be used as fingerprint for standardization of traditional medicine extractcontainingPiper retrofractum Vahl. Fructus.
Key words: Piper retrofractum Vahl.fructus, fingerprint, TLC scanner, retention factor.
Presented at 10th Asian Pasific Pharmaceutical Symposium. Yogyakarta, Indonesia, 04 July 2011