By: Waty TD1, Yarni L1, Murhandini S1, and Rahayu WP2
1Research Center for Drug and Food, National Agency for Drug and Food Control,
2Department of Food Science and Technology, Bogor Agricultural University
Zingiber officinale var rubrum is used in folk medicine as carminative, stimulant of the gastro intestinal tract and counter irritant. The aim of study was to obtain the fingerprint through chromatogram profiles of Zingiber officinale var rubrum that can be used for standardizing of traditional medicines. This research was carried out by material preparation and extraction from its crude extract in various solvents i.e. n-hexane, chloroform, ethyl acetate, and ethanol, then followed by identification using TLC with scanner and documentary system in reliable resolutions at UV λ 254 and λ 366 nm. Analysis by HPLC was also performed to find chromatogram profile with specific retention time. From the results of TLC, elution in n–hexane: diethyl ether (45:55 v/v), all extracts showed a similar pattern with specific retention factors at about 0.12 and 0.28 (254 nm) and 0.19 (366 nm). Otherwise, elution in toluene: diethyl ether: ethyl acetate (60:30:10 v/v/v), all extracts had different pattern with specific retention factor at about 0.02 and 0.31 (254 nm). Analysis by HPLC using column C18 and mobile phases: (A) acetonitrile: water: ammonium acetate 2 % (59:39:2 v/v/v) and (B) acetonitrile: ammonium acetate 2 % (98:2 v/v) respectively resulted the best profile chromatogram for the n-hexane, ethyl acetate and ethanol extracts. In conclusion, the chromatogram profiles obtained can be used as fingerprint for standardization of Zingiber officinale var rubrum extract.
Keywords: Zingiber officinale var rubrum rhizome, TLC scanner, fingerprint, chromatogram
Presented at International Conference on Natural Products. University Putra Malaysia. Kuala Lumpur Malaysia, 13-16 November 2011