[abstract] ANALYTICAL PROTOCOL FOR DETERMINATION ALPHA MANGOSTIN AS Garcinia mangostana L. MARKER USING HPLC

source: pribadi

By: Siregar MN1, Nasrullah I1, Murhandini S1, Rahayu WP12

1Research Center for Drug and Food, National Agency for Drug and Food Control
2Department of Food Science and Technology, Bogor Agricultural University

Garcinia mangostana L., hull part,is empirically used as a traditional medicinefor diarrhea, sprue and antipyretic. Its chemical constituents are xanthon compounds such asa-mangostin, 8-deoxygartanin, gartanin, mangostinone, tovophylin A and cudraxanthone. Eventhough a-mangostin is the major compound, however, there is little information for quality and quantity determination of α-mangostin in mangosteen. Thus, were the aims of this study. The other purpose was to set up a validated method (ICH Harmonized Tripartite Guideline, 2005) for quality control and quantity determination of a-mangostin from Garcinia mangostana L. using reverse-phase HPLC. The method was validated and showed to be linear (r= 0.999), accurate (mean 100.228%), precise (intra-day variation = 0.835%, inter-day variation = 0.635%), specific with good recovery (mean 100,228 %). Total analysis was nearly 5.4 min. The method is applicable for standardization and for routine quality control of α-mangostin in Garcinia mangostana L. hull extract and its products.

Keywords:Garcinia mangostana L., hull, a-mangostin, reverse-phase, HPLC

Presented at International Conference 2ndBiennial International Conference on New Developments in Drug Discovery from Natural Products and Traditional Medicines, Punjab-India, November 20-24th 2010

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