By: Hartuti W1, Pusparini N1, Panggabean RIL1, Rahayu WP1,2

1Research Center for Drug and Food, National Agency for Drug and Food Control
2Department of Food Science and Technology, Bogor Agricultural University

Many contaminants can contaminate food and cause product unsafe to consume. One of the emerging food contaminant is packaging component such as DEHP (Di-(2-ethylhexyl phthalate)) that can migrate into food matrices. These components mainly used as plasticizers and included in the second categories of food labeling and hazard classification.There were three steps for this research: determination of instrument’s (Gas Chromatography-Mass Spectrometer (GC-MS)) detection limit (IDL), DEHP determination method from food packaging materials, and DEHP migration test into food stimulant. N-heptane (38oC) was used as food stimulant to extract DEHP from samples in 30 minutes. Food stimulant was used to represent the matrices of fat and oily foods. The phthalate level determination used a modified method from Consumer Product Safety Commission (CPSC) with the addition of miliQ. This method used tetrahydrofuran to dissolve the polar fraction of PVC samples, and n-hexane to separate between polar and non polar molecules. The later addition of miliQ was intended to fasten the separation’s time. There were three method to be tested: CPSC-1 (without miliQ), CPSC-2 (added with 5 ml miliQ), and CPSC-3 (added with 10 ml miliQ). Then, to test sixteen samples of PVC, method from EU (2001) combined method from Badan POM RI (2007) were used. Instrument’s detection limit was the lower concentration limit of DEHP in n-heptane could be quantified by GC-MS. The IDL value for this method was 1.00 µg/ml n-heptane. DEHP percentages for three modification methods were 2.83; 119.77; and 40.74% respectively. Having got the result, CPSC-3 was used as a recommended method. Two samples were not containing DEHP, and DEHP migration scores for the other fourteen samples ranged between 1.56 to 25.52 μg/min/10 cm2. Referring to the CSTEE (EU Scientific Committee on Toxicity, Ecotoxicity and the Environment), the allowed level of DEHP is 1.67 μg/min/10 cm2.

Keywords: phthalate, packaging, migration, GC-MS

Presented at International Seminar Emerging Issues and Technology Development in Food and Ingredients, Jakarta, September 29-30th 2010.


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