[abstrak] REKAYASA MEDIUM FERMENTASI UNTUK PRODUKSI L-LISIN OLEH Corynebacterium glutamicum ATCC 21513 DENGAN MENGGUNAKAN MOLASSES SEBAGAI SUMBER KARBON (FERMENTATION MEDIUM MODIFIED TO PRODUCE L-LYSINE BY Corynebacterium glutamicum ATCC 21513 USING MOLASSES AS SOURCE OF CARBON)

By: Dasarani DD, Rahayu WP , Satiawihardja B

Department of Food Science and Technology, Bogor Agricultural University

Corynebacterium glutamicum ATCC 21513 is one of mutant strains that is made for producing a large amount of L-lysine. This strain has homoserine auxotroph character (double auxotroph i.e. menthionin and threonin), leucine auxotroph, and penicillin resistance. In mutant auxotroph, L-lysine production will increase if essential nutrients are included into their fermentation media. In this study, an effort was made to increase L-lysine production by adding certain amount of methionine, threonin, and leucine, which was intended to enrich such amino acids contained in pepton, into a medium. For changing cell wall permeability, penicillin was added into fermentation media to release L-lysine from the cells and resulted in L-lysine accumulation.

Keywords:fermentation, L-lysine, Corynebacterium glutamicum, molasses

Dimuat pada Jurnal Agroland, Volume 8 No.1, 2001, ISSN 0854-641X

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