[abstrak] EKTRAKSI DAN FRAKSINASI KOMPONEN ANTIMIKROBA DARI RIMPANG LENGKUAS (Alpinia galangal L. SWARTZ) (EXTRACTION AND FRACTIONATION OF ANTIMICROBIAL COMPONENTS FROM GALANGAL (Alpinia galangal L. SWARTZ))

By: Rahayu WP1, Fardiaz S1,  Jenie BSL1, Satiawihardja B1, Apriyantono A1,  Darusman LK2

1Department of Food Science and Technology, Bogor Agricultural University
2Department of Chemistry, Bogor Agricultural University

Ground galangal was extracted and fractionated with n-hexane, methanol, chloroform-water and distillated with steam to isolate the antimicrobial fractions. The antimicrobial activity was determined by agar diffusion method against spore forming bacteria (B. cereus), Gram-positive bacteria (S. aureus and L. monocytogenes), Gram-negative bacteria (V. cholerae and P. aeruginosa) and mold (R. oligosporus and A. flavus). The chloroform extract was the most effective, successively followed by extract of methanol, n-hexane and essential oil. S. aureus and B. cereus were the most sensitive bacteria than other tested bacteria. Antimicrobial activities of the fraction obtained from silica gel column chromatography indicated that fraction 3 contained acetoxycavicol acetate as the main compound.

Keywords: antimicrobial, galangal, extraction

Dipresentasikan pada Seminar Nasional Pangan, Yogyakarta-Indonesia, September 14th 1999. Proceeding ISBN: 979-95781-0-8

 

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